BATLAS: Deconvoluting Brown Adipose Tissue
Sven Enerbäck; Pirjo Nuutila; Holger Klein; Markku Taittonen; Nassila Akchiche; Sebastian Müller; Aliki Perdikari; Hua Dong; Jozef Ukropec; Tarja Niemi; Francesc Fernandez-Albert; Nuno D. Pires; Miroslav Balaz; Lucia Balazova; Patrik Stefanicka; Martin E. Lidell; Christian Wolfrum; Germán Gastón Leparc; Wenfei Sun; Kirsi A. Virtanen; Lennart Opitz; Gottfried Rudofsky; Heike Neubauer; Lukas Varga; Elia Stupka; Eva Röder
BATLAS: Deconvoluting Brown Adipose Tissue
Sven Enerbäck
Pirjo Nuutila
Holger Klein
Markku Taittonen
Nassila Akchiche
Sebastian Müller
Aliki Perdikari
Hua Dong
Jozef Ukropec
Tarja Niemi
Francesc Fernandez-Albert
Nuno D. Pires
Miroslav Balaz
Lucia Balazova
Patrik Stefanicka
Martin E. Lidell
Christian Wolfrum
Germán Gastón Leparc
Wenfei Sun
Kirsi A. Virtanen
Lennart Opitz
Gottfried Rudofsky
Heike Neubauer
Lukas Varga
Elia Stupka
Eva Röder
CELL PRESS
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042720103
https://urn.fi/URN:NBN:fi-fe2021042720103
Tiivistelmä
Recruitment and activation of thermogenic adipocytes have received increasing attention as a strategy to improve systemic metabolic control. The analysis of brown and brite adipocytes is complicated by the complexity of adipose tissue biopsies. Here, we provide an in-depth analysis of pure brown, brite, and white adipocyte transcriptomes. By combining mouse and human transcriptome data, we identify a gene signature that can classify brown and white adipocytes in mice and men. Using a machine-learning-based cell deconvolution approach, we develop an algorithm proficient in calculating the brown adipocyte content in complex human and mouse biopsies. Applying this algorithm, we can show in a human weight loss study that brown adipose tissue (BAT) content is associated with energy expenditure and the propensity to lose weight. This online available tool can be used for in-depth characterization of complex adipose tissue samples and may support the development of therapeutic strategies to increase energy expenditure in humans.
Kokoelmat
- Rinnakkaistallenteet [19207]