Construction and Characterization of Synthetic Bacterial Community for Experimental Ecology and Evolution
Tamminen M; Cairns J; Virta M; Jokela R; Hiltunen T; Hultman J
Construction and Characterization of Synthetic Bacterial Community for Experimental Ecology and Evolution
Tamminen M
Cairns J
Virta M
Jokela R
Hiltunen T
Hultman J
FRONTIERS MEDIA SA
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042719683
https://urn.fi/URN:NBN:fi-fe2021042719683
Tiivistelmä
Experimental microbial ecology and evolution have yielded foundational insights into ecological and evolutionary processes using simple microcosm setups and phenotypic assays with one- or two-species model systems. The fields are now increasingly incorporating more complex systems and exploration of the molecular basis of observations. For this purpose, simplified, manageable and well-defined multispecies model systems are required that can be easily investigated using culturing and high-throughput sequencing approaches, bridging the gap between simpler and more complex synthetic or natural systems. Here we address this need by constructing a completely synthetic 33 bacterial strain community that can be cultured in simple laboratory conditions. We provide whole-genome data for all the strains as well as metadata about genomic features and phenotypic traits that allow resolving individual strains by amplicon sequencing and facilitate a variety of envisioned mechanistic studies. We further show that a large proportion of the strains exhibit coexistence in co-culture over serial transfer for 48 days in the absence of any experimental manipulation to maintain diversity. The constructed bacterial community can be a valuable resource in future experimental work.
Kokoelmat
- Rinnakkaistallenteet [19207]