dc.contributor.author | Palvimo JJ | |
dc.contributor.author | Brose N | |
dc.contributor.author | Daniel JA | |
dc.contributor.author | Zhang FP | |
dc.contributor.author | Tirard M | |
dc.contributor.author | Cooper BH | |
dc.date.accessioned | 2022-10-28T13:07:04Z | |
dc.date.available | 2022-10-28T13:07:04Z | |
dc.identifier.uri | https://www.utupub.fi/handle/10024/162911 | |
dc.description.abstract | SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and immunostaining analyses of synaptic SUMO1-conjugation. By using a knock-in reporter mouse line expressing tagged SUMO1, we could not detect SUMO1-conjugation of seven previously proposed synaptic SUMO1-targets in the brain. Further, immunostaining of cultured neurons from wild-type and SUMO1 knock-out mice showed that anti-SUMO1 immunolabelling at synapses is non-specific. Our findings indicate that SUMO1-conjugation of synaptic proteins does not occur or is extremely rare and hence not detectable using current methodology. Based on our data, we discuss a set of experimental strategies and minimal consensus criteria for the validation of SUMOylation that can be applied to any SUMOylation substrate and SUMO isoform. | |
dc.language.iso | en | |
dc.publisher | ELIFE SCIENCES PUBLICATIONS LTD | |
dc.title | Analysis of SUMO1-conjugation at synapses | |
dc.identifier.url | https://research.utu.fi/converis/portal/Publication/26330217 | |
dc.identifier.urn | URN:NBN:fi-fe2021042717108 | |
dc.relation.volume | 6 | |
dc.contributor.organization | fi=biolääketieteen laitos, yhteiset|en=Institute of Biomedicine| | |
dc.contributor.organization-code | 2607100 | |
dc.converis.publication-id | 26330217 | |
dc.converis.url | https://research.utu.fi/converis/portal/Publication/26330217 | |
dc.format.pagerange | 36 | |
dc.format.pagerange | 1 | |
dc.identifier.jour-issn | 2050-084X | |
dc.okm.affiliatedauthor | Zhang, Fuping | |
dc.okm.discipline | 3111 Biomedicine | en_GB |
dc.okm.discipline | 3111 Biolääketieteet | fi_FI |
dc.okm.internationalcopublication | international co-publication | |
dc.okm.internationality | International publication | |
dc.okm.type | Journal article | |
dc.publisher.country | Britannia | fi_FI |
dc.publisher.country | United Kingdom | en_GB |
dc.publisher.country-code | GB | |
dc.relation.articlenumber | ARTN e26338 | |
dc.relation.doi | 10.7554/eLife.26338 | |
dc.relation.ispartofjournal | eLife | |
dc.year.issued | 2017 | |