Näytä suppeat kuvailutiedot

Analysis of SUMO1-conjugation at synapses

Palvimo JJ; Brose N; Daniel JA; Zhang FP; Tirard M; Cooper BH

dc.contributor.authorPalvimo JJ
dc.contributor.authorBrose N
dc.contributor.authorDaniel JA
dc.contributor.authorZhang FP
dc.contributor.authorTirard M
dc.contributor.authorCooper BH
dc.date.accessioned2022-10-28T13:07:04Z
dc.date.available2022-10-28T13:07:04Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/162911
dc.description.abstractSUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and immunostaining analyses of synaptic SUMO1-conjugation. By using a knock-in reporter mouse line expressing tagged SUMO1, we could not detect SUMO1-conjugation of seven previously proposed synaptic SUMO1-targets in the brain. Further, immunostaining of cultured neurons from wild-type and SUMO1 knock-out mice showed that anti-SUMO1 immunolabelling at synapses is non-specific. Our findings indicate that SUMO1-conjugation of synaptic proteins does not occur or is extremely rare and hence not detectable using current methodology. Based on our data, we discuss a set of experimental strategies and minimal consensus criteria for the validation of SUMOylation that can be applied to any SUMOylation substrate and SUMO isoform.
dc.language.isoen
dc.publisherELIFE SCIENCES PUBLICATIONS LTD
dc.titleAnalysis of SUMO1-conjugation at synapses
dc.identifier.urlhttps://research.utu.fi/converis/portal/Publication/26330217
dc.identifier.urnURN:NBN:fi-fe2021042717108
dc.relation.volume6
dc.contributor.organizationfi=biolääketieteen laitos, yhteiset|en=Institute of Biomedicine|
dc.contributor.organization-code2607100
dc.converis.publication-id26330217
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/26330217
dc.format.pagerange36
dc.format.pagerange1
dc.identifier.jour-issn2050-084X
dc.okm.affiliatedauthorZhang, Fuping
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryBritanniafi_FI
dc.publisher.countryUnited Kingdomen_GB
dc.publisher.country-codeGB
dc.relation.articlenumberARTN e26338
dc.relation.doi10.7554/eLife.26338
dc.relation.ispartofjournaleLife
dc.year.issued2017


Aineistoon kuuluvat tiedostot

Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot