Prolongation of H2 photoproduction by immobilized, sulfur-limited Chlamydomonas reinhardtii cultures

Abstract

Two approaches to prolong the duration of hydrogen production by immobilized, sulfur-limited Chlamydomonas reinhardtii cells are examined. The results demonstrate that continuous H(2) photoproduction can occur for at least 90 days under constant flow of TAP medium containing micromolar sulfate concentrations. Furthermore, it is also possible to prolong the duration of H(2) production by cycling immobilized cells between minus and plus sulfate conditions. (C) 2008 Elsevier B.V. All rights reserved.