Breeze: an integrated quality control and data analysis application for high-throughput drug screening
John-Patrick Mpindi; Swapnil Potdar; Olli Kallioniemi; Philipp Ianevski; Krister Wennerberg; Bhagwan Yadav; Aleksandr Ianevski; Päivi Östling; Tero Aittokallio; Dmitrii Bychkov; Clément Fiere; Jani Saarela
Breeze: an integrated quality control and data analysis application for high-throughput drug screening
John-Patrick Mpindi
Swapnil Potdar
Olli Kallioniemi
Philipp Ianevski
Krister Wennerberg
Bhagwan Yadav
Aleksandr Ianevski
Päivi Östling
Tero Aittokallio
Dmitrii Bychkov
Clément Fiere
Jani Saarela
OXFORD UNIV PRESS
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042822004
https://urn.fi/URN:NBN:fi-fe2021042822004
Tiivistelmä
High-throughput screening (HTS) enables systematic testing of thousands of chemical compounds for potential use as investigational and therapeutic agents. HTS experiments are often conducted in multi-well plates that inherently bear technical and experimental sources of error. Thus, HTS data processing requires the use of robust quality control procedures before analysis and interpretation. Here, we have implemented an open-source analysis application, Breeze, an integrated quality control and data analysis application for HTS data. Furthermore, Breeze enables a reliable way to identify individual drug sensitivity and resistance patterns in cell lines or patient-derived samples for functional precision medicine applications. The Breeze application provides a complete solution for data quality assessment, dose-response curve fitting and quantification of the drug responses along with interactive visualization of the results.
Kokoelmat
- Rinnakkaistallenteet [19207]