Site-Specific In-111-Radiolabeling of Dual-PEGylated Porous Silicon Nanoparticles and Their In Vivo Evaluation in Murine 4T1 Breast Cancer Model
Dave Lumen; Surachet Imlimthan; Mirkka Sarparanta; Elisavet Lambidis; Wujun Xu; Vesa-Pekka Lehto; Simo Näkki; Anu J. Airaksinen
Site-Specific In-111-Radiolabeling of Dual-PEGylated Porous Silicon Nanoparticles and Their In Vivo Evaluation in Murine 4T1 Breast Cancer Model
Dave Lumen
Surachet Imlimthan
Mirkka Sarparanta
Elisavet Lambidis
Wujun Xu
Vesa-Pekka Lehto
Simo Näkki
Anu J. Airaksinen
MDPI
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042823319
https://urn.fi/URN:NBN:fi-fe2021042823319
Tiivistelmä
Polyethylene glycol (PEG) has been successfully used for improving circulation time of several nanomaterials but prolonging the circulation of porous silicon nanoparticles (PSi NPs) has remained challenging. Here, we report a site specific radiolabeling of dual-PEGylated thermally oxidized porous silicon (DPEG-TOPSi) NPs and investigation of influence of the PEGylation on blood circulation time of TOPSi NPs. Trans-cyclooctene conjugated DPEG-TOPSi NPs were radiolabeled through a click reaction with [In-111]In-DOTA-PEG(4)-tetrazine (DOTA = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) and the particle behavior was evaluated in vivo in Balb/c mice bearing 4T1 murine breast cancer allografts. The dual-PEGylation significantly prolonged circulation of [In-111]In-DPEG-TOPSi particles when compared to non-PEGylated control particles, yielding 10.8 +/- 1.7% of the injected activity/g in blood at 15 min for [In-111]In-DPEG-TOPSi NPs. The improved circulation time will be beneficial for the accumulation of targeted DPEG-TOPSi to tumors.
Kokoelmat
- Rinnakkaistallenteet [19207]