Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
Yoshikazu Takada; Jyrki Heino; Linda Sandblad; Lars Frängsmyr; Annelie Olofsson; Lynn M. Schnapp; Jason Gall; Niklas Arnberg; Anandi Rajan; A. Paul Mould; B. David Persson
Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
Yoshikazu Takada
Jyrki Heino
Linda Sandblad
Lars Frängsmyr
Annelie Olofsson
Lynn M. Schnapp
Jason Gall
Niklas Arnberg
Anandi Rajan
A. Paul Mould
B. David Persson
NATURE PUBLISHING GROUP
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042719432
https://urn.fi/URN:NBN:fi-fe2021042719432
Tiivistelmä
The enteric species F human adenovirus types 40 and 41 (HAdV-40 and -41) are the third most common cause of infantile gastroenteritis in the world. Knowledge about HAdV-40 and -41 cellular infection is assumed to be fundamentally different from that of other HAdVs since HAdV-40 and -41 penton bases lack the aV-integrin-interacting RGD motif. This motif is used by other HAdVs mainly for internalization and endosomal escape. We hypothesised that the penton bases of HAdV-40 and -41 interact with integrins independently of the RGD motif. HAdV-41 transduction of a library of rodent cells expressing specific human integrin subunits pointed to the use of laminin-binding alpha 2-, alpha 3- and alpha 6- containing integrins as well as other integrins as candidate co-receptors. Specific laminins prevented internalisation and infection, and recombinant, soluble HAdV-41 penton base proteins prevented infection of human intestinal HT-29 cells. Surface plasmon resonance analysis demonstrated that HAdV-40 and -41 penton base proteins bind to alpha 6-containing integrins with an affinity similar to that of previously characterised penton base:integrin interactions. With these results, we propose that laminin-binding integrins are co-receptors for HAdV-40 and -41.
Kokoelmat
- Rinnakkaistallenteet [19207]