Pim-1 kinase promotes inactivation of the pro-apoptotic bad protein by phosphorylating it on the Ser(112) gatekeeper site
Peltola KJ; Mankonen HP; Lilly M; Koskinen PJ; Sandholm J; Aho TLT
Pim-1 kinase promotes inactivation of the pro-apoptotic bad protein by phosphorylating it on the Ser(112) gatekeeper site
Peltola KJ
Mankonen HP
Lilly M
Koskinen PJ
Sandholm J
Aho TLT
ELSEVIER SCIENCE BV
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042718111
https://urn.fi/URN:NBN:fi-fe2021042718111
Tiivistelmä
Constitutive expression of the Pim-1 kinase prolongs survival of cytokine-deprived FDCP1 cells, partly via maintenance of Bcl-2 expression. Here, we show that Pim-1 colocalizes and physically interacts with the pro-apoptotic Bad protein and phosphorylates it in vitro on serine 112, which is a gatekeeper site for its inactivation. Furthermore, wild-type Pim-1, but not a kinase-deficient mutant, enhances phosphorylation of this site in FDCP1 cells and protects cells from the pro-apoptotic effects of Bad. Our results suggest that phosphorylation of Bad by Pim-1 is one of several mechanisms via which the Pim-1 kinase can enhance Bcl-2 activity and promote cell survival. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Kokoelmat
- Rinnakkaistallenteet [19207]