In Vitro Drug Screening of Cell Culture Models for LMNA-Related Dilated Cardiomyopathy
Turunen, Minttu (2023-05-11)
In Vitro Drug Screening of Cell Culture Models for LMNA-Related Dilated Cardiomyopathy
(11.05.2023)
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
suljettu
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2023062057142
https://urn.fi/URN:NBN:fi-fe2023062057142
Tiivistelmä
Mutations in the LMNA gene encoding lamins A and C cause inherited diseases commonly called
laminopathies. The most common laminopathy is dilated cardiomyopathy (DCM) and currently, there
are no specific treatments for the disease. This study aimed to identify drugs that affect cell viability
and morphology of lamin mutant cells. Dermal fibroblasts from a DCM patient carrying the
p.S143P-LMNA mutation and from a healthy donor were screened with 166 drugs using
imaging-based analysis. Based on cell viability, the most interesting drugs were selected for further
testing on neonatal cardiomyocytes isolated from transgenic p.S143P-Lmna mice and on
human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) from DCM-patient and
healthy control.
The effects of the selected drugs (glimepiride, nicorandil, sotagliflozin, 4-PBA and gemcitabine) on
cell viability and lamin A and C protein expression were further investigated after 72-hour
incubations. Additionally, immunofluorescence stainings were used to visualize the lamina structure
(lamin A/C), actin cytoskeleton (F-actin), cardiomyocyte sarcomere structure (a-actinin) and DNA
damage (γH2AX).
Similar results in cell viability could not be reproduced using an ATP-based assay and viability
between isolated neonatal cardiomyocytes from LmnaWT/WT and LmnaS143P/S143P mice remained
unaffected. Also, no obvious damage in the lamina, sarcomere structure or actin cytoskeleton was
detected. Major defects in heterochromatin organization were found in the LmnaS143P/S143P mouse
cardiomyocytes, however, the chosen drugs were unable to restore such changes. Since previous
reports have shown that DCM-linked cellular defects manifest particularly under external stress, it
would be important to investigate the impact of drugs under stressed conditions in the future.
laminopathies. The most common laminopathy is dilated cardiomyopathy (DCM) and currently, there
are no specific treatments for the disease. This study aimed to identify drugs that affect cell viability
and morphology of lamin mutant cells. Dermal fibroblasts from a DCM patient carrying the
p.S143P-LMNA mutation and from a healthy donor were screened with 166 drugs using
imaging-based analysis. Based on cell viability, the most interesting drugs were selected for further
testing on neonatal cardiomyocytes isolated from transgenic p.S143P-Lmna mice and on
human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) from DCM-patient and
healthy control.
The effects of the selected drugs (glimepiride, nicorandil, sotagliflozin, 4-PBA and gemcitabine) on
cell viability and lamin A and C protein expression were further investigated after 72-hour
incubations. Additionally, immunofluorescence stainings were used to visualize the lamina structure
(lamin A/C), actin cytoskeleton (F-actin), cardiomyocyte sarcomere structure (a-actinin) and DNA
damage (γH2AX).
Similar results in cell viability could not be reproduced using an ATP-based assay and viability
between isolated neonatal cardiomyocytes from LmnaWT/WT and LmnaS143P/S143P mice remained
unaffected. Also, no obvious damage in the lamina, sarcomere structure or actin cytoskeleton was
detected. Major defects in heterochromatin organization were found in the LmnaS143P/S143P mouse
cardiomyocytes, however, the chosen drugs were unable to restore such changes. Since previous
reports have shown that DCM-linked cellular defects manifest particularly under external stress, it
would be important to investigate the impact of drugs under stressed conditions in the future.